Violet 3.0

Simplifying FLIM/FRET to empower life science research.

Violet 3.0 is the all-in-one microscopy platform for QF-Pro® technology, bringing Fluorescence Lifetime Imaging Microscopy (FLIM) and Förster Resonance Energy Transfer (FRET) to any lab. Our comprehensive benchtop solution features a high-end modulated laser, sensitive image sensor, and also a multi-channel LED for immunofluorescence. Violet 3.0 includes two stage inserts: one for up to 4 microscopy slides and another for a standard multi-well plate, accommodating various sample types. Its ease of use supports both experienced scientists and beginners in exploring spatial biology with confidence. Experience research as never before and pave the way for groundbreaking advancements in spatial biology. Violet 3.0 – where simplicity meets innovation, opening whole new avenues of research.

Product features

Works with plates or slides

Violet 3.0 has an easy to switch stage insert, allowing for the analysis of microwell plates or up to four standard microscope slides at a time. It is equipped with 10X, 20X and 40X dry objectives.

Easy to use software

Violet 3.0 is accompanied with one desktop licence of our QF-Pro® software allowing users of all expertise to easily run the device and generate groundbreaking data.

Includes 4 LED channels

Alongside the modulated laser required for QF-Pro® analysis a secondary multichannel LED (365nm, 488nm, 585nm and 635nm) is included for the illumination of secondary biomarkers of interest.

QF-Pro® Software

What makes Violet 3.0 even more accessibleand easy to use is the accompanying QF-Pro® software suite. This guides users of all experience through the process of loading their samples and creating a map of Regions of Interest (ROIs) to be acquired. The platform then sequentially acquires all mapped ROIs and calculates a QF-Pro® score and image per region. Post-acquisition analyses, such as the application of a threshold or change in Lookup Table (LUT) is automated, with the option of fine-tuning for more experienced users. All data can then be seamlessly exported from the QF-Pro® software into Microsoft Excel (data) or PowerPoint/PDF (images). The combination of Violet 3.0 and QF-Pro® software allows any laboratory or researcher to access to benefits of QF-Pro®.

QF-Pro® reagent kits

Discover our flexible reagent kits which allow QF-Pro® to be used in any laboratory setting. Our kits contain everything needed for a user to perform QF-Pro® analytics in house and can be applied to any biomarker.

Validated Biomarkers

Find out more about the wide range of validated QF-Pro® assays that we offer, ranging from immune checkpoint interactions to dimerisations or kinase activation states. Our flexible technology can be applied to quantify the function of any biomarker in any disease setting.